This Shiny App can be used to plot data from the manuscript 'Functional profiling of the Toxoplasma genome during acute mouse infection.'
Each tab allows you to generate a plot highlighting a gene of interest from the data sets generated in this study.

Citation: Giuliano, Christopher J et al. “Functional profiling of the Toxoplasma genome during acute mouse infection.”
bioRxiv : the preprint server for biology 2023.03.05.531216. 6 Mar. 2023, doi:10.1101/2023.03.05.531216. Preprint.

Fitness across tissues

Differential fitness is calculated as the distance of the observed tissue fitness score relative to a predicted regression line to cell culture fitness.
The differential fitness composite scores take into account both the magnitude of differential fitness and statistical significance.

We consider differential phenotype scores >30 or <-30 to be significant.
Genes that are fitness-conferring (scores <-5) in cell culture are colored in orange.
Gene of interest will appear in blue.

Hover-over plot for mouse peritoneum differential fitness

Differential fitness is calculated as the distance of the observed tissue fitness score relative to a predicted regression line to cell culture fitness.
The differential fitness composite scores take into account both the magnitude of differential fitness and statistical significance.

We consider differential phenotype scores >30 or <-30 to be significant.
Genes that are fitness-conferring (scores <-5) in cell culture are colored in orange.
Gene of interest will appear in blue.

Delayed defects in extended cell culture

Generate plots comparing gene fitness scores at passage 4 and passage 10.
Hover over points on the plot to see Gene IDs.
Gene of interest will appear in blue.

Cell culture fitness scores in HFFs

Generate plots of gene fitness scores from HFF cell culture screens completed with 9-gRNA and 3-gRNA libraries.
Gene of interest will appear in blue.

Fitness scores in MEF cell lines

Generate plots of gene fitness scores in immortalized MEF cell lines (C57BL/6J and CAST) compared to fitness scores in HFFs.
Gene of interest will appear in blue.